DNA

Part:BBa_K4663025:Design

Designed by: Lorenzo da Silva Saraiva   Group: iGEM23_WageningenUR   (2023-10-12)


SuCas12a2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 646
    Illegal EcoRI site found at 1342
    Illegal EcoRI site found at 1810
    Illegal EcoRI site found at 2281
    Illegal EcoRI site found at 2299
    Illegal EcoRI site found at 2479
    Illegal EcoRI site found at 2623
    Illegal PstI site found at 2125
    Illegal PstI site found at 3513
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 646
    Illegal EcoRI site found at 1342
    Illegal EcoRI site found at 1810
    Illegal EcoRI site found at 2281
    Illegal EcoRI site found at 2299
    Illegal EcoRI site found at 2479
    Illegal EcoRI site found at 2623
    Illegal PstI site found at 2125
    Illegal PstI site found at 3513
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 646
    Illegal EcoRI site found at 1342
    Illegal EcoRI site found at 1810
    Illegal EcoRI site found at 2281
    Illegal EcoRI site found at 2299
    Illegal EcoRI site found at 2479
    Illegal EcoRI site found at 2623
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 646
    Illegal EcoRI site found at 1342
    Illegal EcoRI site found at 1810
    Illegal EcoRI site found at 2281
    Illegal EcoRI site found at 2299
    Illegal EcoRI site found at 2479
    Illegal EcoRI site found at 2623
    Illegal PstI site found at 2125
    Illegal PstI site found at 3513
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 646
    Illegal EcoRI site found at 1342
    Illegal EcoRI site found at 1810
    Illegal EcoRI site found at 2281
    Illegal EcoRI site found at 2299
    Illegal EcoRI site found at 2479
    Illegal EcoRI site found at 2623
    Illegal PstI site found at 2125
    Illegal PstI site found at 3513
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1239
    Illegal BsaI.rc site found at 3436


Design Notes

No start codon



Source

N/A

References